Vol 9 Issue 4 October 2021-December 2021

Abstract: The continuous activities of companies that deal with substances such as hydrocarbons and their derivatives in the environment are the primary source of pollution in our ecosystem, and they must be eliminated and managed. Biocatalysts secreted by Saccharomyces cerevisiae, Bacillus cereus, and Pseudomonas aeruginosa were identified and characterized in this study. The oil-polluted environment is an ideal habitat for isolating lipase-producing microorganisms. At the University of Port Harcourt, Aluu, and Rupkokwu were chosen as case studies because of their distinctive oily environments for isolating lipase-producing microorganisms. The goal of this investigation was to isolate, screen, identify, and characterize lipase-producing fungi and bacteria from oil-contaminated soil. While screening, inoculation on the spot for primary screening, and Lipase synthesis in submerged fermentation were used. To test the effect of temperature on lipase activity, the reaction was carried out at temperatures ranging from 10 to 90 °C. Pseudomonas aeruginosa produced the most lipase, followed by Bacillus cereus, with a minor amount of lipase in the shape of a zone around the Saccharomyces cerevisiae colony. Pseudomonas aeruginosa exhibited the greatest enzyme activity of 17.19 U/L after 72 hours of inoculation into the culture medium, and the biocatalyst producing temperature conditions were maximized at 37^oC. The enzyme activities of the three isolates were 0.5 U/L after 96 hours of inoculation. The lipase was extremely stable in terms of temperature and organic solvent. This one-of-a-kind feature makes it appealing and practical for industrial applications.

Keywords:  Bacillus cereus, Biocatalyst, Biomaterial, Environment, Enzymes, Pseudomonas aeruginosa, Saccharomyces cerevisiae.

Title: ISOLATION, SCREENING, AND IDENTIFICATION OF BIOCATALYST FROM BACTERIA AND FUNGI IN OIL POLLUTED SOIL

Author: Ubong U.Isang, Gideon C. Okpokwasili, Francis Ire

International Journal of Engineering Research and Reviews

ISSN 2348-697X (Online)

Research Publish Journals

Abstract: The oil and gas industry is in need of more practical approach to support the understanding of liquid loading in gas wells in order to aid in the monitoring and solutions to the challenges posed by liquid loading in gas wells. In this paper, a practical facility ‘’UAC Excellence flow loop’’ for liquid loading experiment has been built, and used to study liquid loading concept on 0.37 inch tubing (Figure 1). Three stages of flow regimes have been achieved after 900 seconds of production of multiphase fluids (LPG and Water) (Figure 2). It was observed that downhole temperature is a critical factor to gas well loading. Liquid loading affect gas well pressure quicker at the onset, but gradually affect production rate (Figure 3). Annular flow was obtained at 300 seconds (Figure 5), mist flow at 600 seconds (Figure 6) and Slug flow at 900 seconds (Figure 7).The UAC Excellence flow loop, the first in Nigeria, will serve as practical equipment for the study of liquid loading in smaller diameter tubings

Keywords: Liquid loading, Tubing, UAC Excellence Flow loop, Downhole.

Title: INVESTIGATION OF LIQUID LOADING CONCEPT IN 0.37 INCH TUBING USING UAC EXCELLENCE FLOW LOOP

Author: Udoaka, Mfon Godwill,  Dulu, Appah,  Joseph, Amiebimbama

International Journal of Engineering Research and Reviews

ISSN 2348-697X (Online)

Research Publish Journals