Vol 14 Issue 1 January 2026-March 2026

Abstract: This study aimed to produce and compare the mosquito repellent properties of lemongrass (Cymbopogon citratus), basil (Ocimum basilicum), and lemonsito (Citrus macrocarpa) leaf extracts against Culex pipiens mosquitoes. The research utilized an experimental-comparative design conducted under controlled laboratory conditions. The plant leaves were extracted using 95% ethyl alcohol through maceration and prepared into spray solutions at concentrations of 5%, 10%, and 15%, with distilled water (0%) as the control. Repellent effectiveness was measured through the percentage of mosquito displacement. Results showed that all extracts exhibited repellent properties, with increasing effectiveness at higher concentrations. At 15% concentration, lemongrass recorded the highest displacement (86.67%), followed by lemonsito (83.33%) and basil (76.67%). Based on the average displacement, lemongrass ranked first (74.45%), lemonsito second (72.22%), and basil third (67.78%). The findings indicate that lemongrass extract is the most effective natural mosquito repellent among the three. The study concludes that plant-based repellents, particularly lemongrass, can serve as safe, affordable, and eco-friendly alternatives to synthetic chemical repellents.

Keywords: Lemongrass extract, basil extract, lemonsito extract, mosquito repellent, Culex pipiens, plant-based repellent, mosquito displacement.

Title: COMPARATIVE STUDY ON THE MOSQUITO REPELLENT PROPERTIES OF LEMONGRASS, BASIL, AND LEMONSITO LEAF EXTRACTS

Author: Candog, Rhiane T.; Abo-abo, Leann T.; Sabsal, Charline P.; Libardo, Janine E.; Rose, Victor P.; Manliguez, James B.

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 31-32

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Website: www.researchpublish.com

Published Date: 25-March-2026

Abstract: The popularity of exotic pets in Thailand is growing quite fast, especially among teenagers, and keeping pets is not only dogs and cats anymore. The number of exotic animals being kept as pets has increased in recent years. Many Thai teenagers are becoming interested in animals such as reptiles, rabbits, hamsters, fish, sugar gliders, and hedgehogs because they see them as unique and suitable for modern lifestyles. This study was conducted to explore the factors influencing Thai teenagers’ interest in keeping exotic pets. A survey was collected from 100 Thai teenagers 10-21 years old. The results show that although 52% of respondents do not currently own any pets, 69% would like to have an exotic pet in the future. Small exotic mammals (27%) and reptiles (21%) were the most popular choices. Social media was found to be the biggest factor (75%), followed by family support (61%) and cost considerations (51%). However, some teenagers are still not interested because they worry about expenses, safety risks, and limited access to veterinary services. Even so, overall interest continues to increase, and this trend is expected to grow further in the future as information and awareness about exotic pets continue to expand.

Keywords: exotic pets, exotic animals, Thai teenagers, veterinary services, factors influencing.

Title: Factors Influencing Thai Teenagers’ Interest in Keeping Exotic Pets

Author: Tiyada Yooyen

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 27-30

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Website: www.researchpublish.com

Published Date: 04-March-2026

Abstract: In this study, lipid peroxidation in terms of TBARS formed and antioxidant defence comprising both antioxidant enzymes and non-enzymatic antioxidants were assessed in the liver tissue of common Asian toads during hibernation and compared with the summer active toads. A significant (P<0.001) increase in lipid peroxidation was found in liver tissue during hibernation. Concomitantly significant (P<0.001) increase in catalase activities was also found during hibernation, with an elevated superoxide dismutase activity both during hibernation and the summer active period. Ascorbic acid, a potent non-enzymatic antioxidant, was also found to be augmented during hibernation compared to summer-active toads. However, reduced glutathione (GSH) was found at low levels during hibernation compared to the summer active period. It may be due to its low rate of biosynthesis during the hypo metabolic condition of hibernation. Increased lipid peroxidation, an indication of oxidative stress and augmented antioxidant defence that were found in this investigation, are not only an adaptive response during hibernation but also a preparation for the oxidative burst expected during arousal from hibernation.

Keywords: Hibernation, oxidative stress, Lipid peroxidation, antioxidant enzymes, and non-enzymatic antioxidants.

Title: LIPID PEROXIDATION AND ANTIOXIDANT DEFENCE IN LIVER TISSUE OF HIBERNATING COMMON ASIAN TOADS, Duttaphrynus melanostictus

Author: Sibakalyani Acharya, Debadas Sahoo, Nidhi Goyal

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 16-26

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Website: www.researchpublish.com

Published Date: 26-February-2026

Abstract: Bio-enzyme is a fermented sap of fruits. Locally available fruits was collected from the market and removing the upper layer seeds of their and using pulp of the fruit for the further fermentation. Using pulp of fruits and adding water in the container and adding Jaggery and stay for three month. Than after 3 month fermentation enzyme sap would be prepare for the plants use.  One liter bio-enzyme should be dissolve in 15 liter water and its pore in the plant root of Manilkara zapota with the verbal of 3 to 5 days of two year old plant and observed the after one month. After one month treated plants were reported to have denser chlorophyll as compared to control, bio-enzyme contains gibberellic acid, a plant growth regulator that has been shown to help increase stem elongation and, thus, internode length. Bio-enzyme, increase in fruit numbers. The fruit weight sapota was observed to increase.

Keywords: Bio-enzyme, Pulp, Fermentation, Chlorophyll, Gibberellic acid.

Title: Morphological and physiological effect of Bio-enzyme Activity on Manilkara zapota plant

Author: Sunil Kumar Bourasi, Narendra Khore, Anand Verma

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 13-15

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Website: www.researchpublish.com

Published Date: 18-February-2026

Abstract: The Current Research Deals with the Molecular Characterization of Genus Bychowskyella and Comparison among its Species based on 18S rDNA/rRNA, as earlier studies were only focused on Morphological and Morphometric Features which fails to differentiate accurately the closely related species. Phylogenetic Studies have been made based on Construction of Neighbour Joining and Maximum Parsimony Methods, using the MEGA Software. Vectorbuilder tool used for the calculation of Percentage of GC Content in the Genome. Using the ExpaRNA tool Conserveness in the loops of Secondary Structure are made, and MEME Suite Software is used Lastly for Motif Predictions and Confirmation of the Molecular based Studies. This Study further opens the door for many future Researches in the Areas of Parasitology, which will help in combating many Unknown Diseases possibly linked to the Genus Bychowskyella, Studying Host Parasite Interactions. Thus the entire Research bridges the gap between Morphological Based Studies and Molecular Based Studies, Helping in Accurate and Reliable way to differentiate between the Species within the Genus Bychowskyella with that of other Genus in Monogenea.

Keywords: Genus Bychowskyella, Monogenea, 18S rDNA/rRNA, Phylogenetic Analysis, Secondary Structure, Motif Predictions.

Title: Molecular and Phylogenetic Analysis of Genus Bychowskyella, using the 18S rDNA/18S rRNA for Studying Conserveness in the Genome for Species- Level Differentiations and Uses for the Taxonomy

Author: Sammohan Sameeran Pati, Haren Ram Chiary, Pukhrambam Pushpa Devi, Amit Singh Dhaulaniya

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 7-12

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Website: www.researchpublish.com

Published Date: 09-February-2026

Abstract: Introduction. Hemostasis is essential in first aid and emergency medical care; however, physiological clotting mechanisms are often insufficient for rapid bleeding control in acute or severe injuries. Chitosan, a biopolymer derived from shrimp shell chitin, has demonstrated effective hemostatic properties and represents a low-cost and environmentally sustainable biomaterial. This study aimed to compare the hemostatic efficiency of chitosan–citric acid solutions at different ratios and to develop a chitosan-based hydrogel sheet for potential bleeding-control applications.

Methods. Chitosan was extracted from shrimp shells through demineralization and deacetylation processes. Chitosan solutions were prepared using citric acid at ratios of 1:12, 1:6, and 1:4. Hemostatic efficiency was evaluated in vitro using fresh porcine blood by observing clot formation and measuring the weight of blood clot residue after 10 minutes. Distilled water and citric acid solution served as control groups. Differences among experimental groups were analyzed using the Kruskal–Wallis test. The formulation exhibiting the highest hemostatic performance was subsequently fabricated into a hydrogel sheet using polyvinyl alcohol, polyvinylpyrrolidone, glycerin, and microwave irradiation. 

Results. Significant differences in blood clot residue weight were observed among the experimental groups (p = 0.009). The chitosan–citric acid ratio of 1:12 exhibited the highest mean blood clot weight, indicating superior hemostatic performance compared with other formulations and controls. The selected formulation was successfully fabricated into a stable hydrogel sheet with satisfactory physical properties and handling characteristics.

Conclusion. The hemostatic efficiency of chitosan–citric acid systems is strongly dependent on formulation ratio, with the 1:12 ratio providing optimal performance in vitro. The developed chitosan-based hydrogel sheet demonstrates potential as an effective, safe, and environmentally sustainable hemostatic material. Further in vivo and clinical studies are warranted.

Keywords: Chitosan; Hemostasis; Hydrogel; Shrimp shells; Biomaterials.

Title: Development of Haemostatic Hydrogel Sheets from Chitosan Extracted from Shrimp Shells

Author: Phonrawin Chirdchim, Boonyawee Sujeerakulkrai, Piengpor Kanghae

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Vol. 14, Issue 1, January 2026 - March 2026

Page No: 1-6

Research Publish Journals

Website: www.researchpublish.com

Published Date: 03-February-2026