Abstract: Antimicrobial resistance (AMR) is widely recognized as a silent pandemic, threatening decades of medical progress and complicating the treatment of infectious diseases worldwide. Among the myriad resistance determinants, extended-spectrum β-lactamases (ESBLs) have emerged as a major driver of therapeutic failure in clinical medicine. This study is aimed at determining the pattern of resistance of ESBL-producing enterobacteriaceae isolated from fish collected from integrated poultry-fish farms at Ila Orangun, Osun State, Nigeria. From November 2024 to April 2025, a total of 18 catfish were aseptically and randomly collected from six poultry-fish farms, processed and bacteriologically analysed using standard microbiological techniques. Isolated isolates were conventionally characterized, ESBL-production in them were confirmed via double-disc synergy test, and antibiogram of the confirmed ESBL+ isolates were carried out via Kirby-Bauer disc diffusion method. Out of 47 isolates recovered from the catfish, 2 (4.26%) were ESBL+. The ESBL+ isolates were totally resistance (100%) to the beta-lactam combination, the cephems and the monobactam respectively tested in this study but totally susceptible (100%) to the carbapenem. They were totally (100%) multidrug-resistance (MDR) with 50% of them resisting 5 and 4 classes of antibiotics respectively, and showing 0.6 and 0.4 multiple antibiotic resistance index (MARI) respectively. The results confirm integrated agro-ecosystems as important reservoirs of MDR, ESBL-producing Enterobacterales. Urgent interventions in the farming practices, surveillance, and public education are needed to mitigate MDR, ESBL-producing enterobacteriaceae spread from farms produce to human through the food chain.
Keywords: Enterobacterales, Antimicrobial resistance, Extended-spectrum β-lactamases, Pandemic, Catfish.
Title: ANTIBIOGRAM OF ESBL-PRODUCING ENTEROBACTERIACEAE ISOLATED FROM FISH COLLECTED FROM INTEGRATED POULTRY-FISH FARMS AT ILA ORANGUN, OSUN STATE, NIGERIA
Author: Adeluwoye-Ajayi, Olayemi Amos., Akinnibosun, Faith Iguodala., Akinnibosun, Henry Adewale., Iyekowa, Osaro., Akinnibosun, Adeyemi Osarodion
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 14, Issue 3, July 2026 - September 2026
Page No: 82-94
Research Publish Journals
Website: www.researchpublish.com
Published Date: 08-August-2026
