Abstract: Due to the limited capacity of landfills, cellulosic waste is one of the main components of municipal solid waste has become a serious disposal issue in both developed and developing nations. The task of managing solid waste is crucial and difficult. Newspaper, tissue paper, cardboard, wood powder, and dry leaves, which are cellulosic waste, are becoming a more appealing choice for the generation of bioethanol due to its diminished feedstock costs, increased potential for substituting fossil fuels, and decreased releases of greenhouse gases. The study framework followed three steps which included the isolation of cellulose-degrading microorganisms, acid (1% H2SO4), and/or enzymatic hydrolysis of substrates followed by fermentation by yeast (Saccharomyces cerevisiae). The highest cellulase enzyme activity of isolate BY7 was 2.004±0.03 U/mL. The optimized condition for acid pretreatment was 1% H2SO4 at 121 °C for 30 minutes heating period. The bacterial isolate (BY7) was used for the enzymatic hydrolysis which helps to convert the cellulose into fermentable sugars. The acid and enzymatic hydrolysates were analyzed by the DNS method. The highest yield of sugars from acid and enzymatic hydrolysis was about 11.8 ± 0.01 g/L and 15.44 ± 0.04 g/L respectively from dry leaves. The fermentable sugars were fermented to produce bioethanol using Saccharomyces cerevisiae and yield was estimated using the Dichromate method.
Keywords: Cellulosic waste; Enzyme activity; Acid and enzymatic pretreatments; Hydrolysates; Fermentation.
Title: Bioethanol production from cellulolytic wastes: A comparative study on acid and enzymatic hydrolysis pretreatments for enhanced saccharification and fermentation
Author: Jainish Panchal, Khatik Tulasa, Yesha Master, Shivani Monya, Smita Parekh
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
Vol. 13, Issue 2, April 2025 - June 2025
Page No: 55-65
Research Publish Journals
Published Date: 25-April-2025
