Abstract: Vegetable oil spills are becoming frequent and are potentially more challenging than petroleum hydrocarbon spills. Yeast lipases occupy a place of prominence among biocatalysts and have potential for rent remediation of vegetable oil spills. There is a need for extensive characterization of lipase for the treatment of vegetable oil-polluted sites. This work was carried out to preliminarily characterise the lipase of Cadida parapsilosis.
Candida parapsilosis was screened for lipase production using standard methods. Temperature, pH, ion concentration (NaNO3 and MgSO4), enzyme concentration, nitrogen concentration, substrate concentration, time course and agitation speed were optimised for the lipase activity as well as growth.
Crude enzyme of Candida parapsilosis had the highest lipase activity and growth of 0.4 U/mL and 1.477 mg/mL respectively but when production was optimised higher activity 0.5 U/mL was seen in the use of glucose as substrate, use of ions like (NH4)2SO4 and Nacl and at agitation speed of 120rpm. Different enzyme and substrate concentration which had optimum enzyme activities for Candida parapsilosis (0.5 U/mL), were 0.5 mL and 1.0 % respectively. Ion concentration (NaNO3 and MgSO4) had optimum growth (1.761mg/mL) at 0.05 %.
The enzyme has good potential for the hydrolysis of vegetable oils, which is an important factor in environmental cleanup of vegetable oil spill site.
Keywords: Lipase production, vegetable oil spill, characterisation.
Title: Characterisation of a Yeast Lipase (Candida parapsilosis) from Vegetable Oil Polluted Soil
Author: Popoola, B.M, Onilude, A.A
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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