Abstract: Proteins and other products from bacteria are becoming increasingly relevant in anticancer treatment due to their high specificity and minimal side effects. Azurin is a novel anticancer protein mainly produced from Pseudomonas aeruginosa and some Gram negative bacteria. This study was conducted on ten Pseudomonas isolates from the biodiversity rich Western Ghats region of Kerala, India. Here, after confirming the presence of anticancer compounds using trypan blue exclusion assay on DLA (Daltons Lymphoma Ascites) cell lines, the Pseudomonas aeruginosa isolate showing the highest cytotoxic activity was chosen for further studies. The identity of the organism was confirmed by biochemical and molecular characteristics. The identified isolate has been designated as Pseudomonas aeruginosa SSj and the 16S rRNA partial sequence was deposited in Genbank (accession number KU821118). The presence of azurin encoding (azu) gene in SSj strain is confirmed using PCR amplification and its sequence is deposited in GenBank (KU821119). The partially purified azurin is studied using FTIR spectrum in comparison with azurin from Pseudomonas aeruginosa MTCC2453 and standard azurin (Sigma). The strain SSj is identified as potential azurin producer and a promising nominee for biomedical applications.
Keywords: Cancer, Pseudomonas aeruginosa, Bacterial protein, azurin, FTIR.
Title: Characterization of anticancer compound azurin from indigenous isolate Pseudomonas aeruginosa SSj
Author: Sereena .M.C, Denoj Sebastian
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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