Abstract: Live adult male specimens of Macromia moorei Selys, 1874 of family Macromiidae have been collected from Andretta, Himachal Pradesh (India). Male germ cell chromosomes of the species have been described on the basis of conventional staining, C-banding, silver nitrate staining and sequence specific staining. The species possesses 2n (♂) =25m, as the chromosome number and XO (♂)/XX (♀) type sex determination. Dark terminal C-bands are present on all the autosomal bivalents, while m bivalent shows small terminal C-bands and X chromosome is C-positive throughout the length. Terminal light/dark NOR’s are present on all autosomal bivalents including m bivalent, while X chromosome also possesses terminal NOR. During sequence specific staining, all the autosomal bivalents show prominant terminal DAPI and CMA3 bright regions, while m bivalent possesses less bright DAPI and CMA3 signals and X chromosome possesses both DAPI and CMA3 regions. Linear characterization of chromosomes of Macromia moorei has been done for the first time.
Keywords: Macromiidae, Chromosomes, Odonata, Anisoptera, Conventional Staining, C-banding, Silver nitrate staining, Sequence specific staining.
Title: Cytogenetic characterization of Macromia moorei Selys, 1874 of family Macromiidae (Odonata:Anisoptera) from India by C-banding, silver nitrate staining and sequence specific staining
Author: Gurinder Kaur Walia, Sarabjit Singh Chahal
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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