Extraction and Detection of Antimicrobial Compound from Streptomyces Filamentosus

Sujatha.Taidala, P.Sivaraagini, M.V. Raman,Vijayalakshmi.Kondaveeti

Abstract: Streptomyces members are well known source of bio control agents due to their ability to produce various secondary metabolites like antibiotics and enzymes. In the present study, a soil actinomycetes member Streptomyces filamentosuswas studied for its ability to produce antimicrobial compounds under controlled conditions. Optimum conditions such as type of medium, pH, temperature, incubation time, salt concentration, carbon and nitrogen source etc., for the production of antimicrobial compounds was determined. It was observed that it can produce two major antibiotic substances one resembles streptomycin (C21H39N7O12) and another was near dioxolomycin(C9H13NO4). In the present study the conditions for the maximum production and the process of production, extraction was also studied. The structural details were determined using NMR, Mass spectroscopic and HPLC analysis. Keywords: Secondary metabolites, compounds, NMR, Mass Spectroscopy and HPLC. Title: Extraction and Detection of Antimicrobial Compound from Streptomyces Filamentosus, Author: Sujatha.Taidala, P.Sivaraagini, M.V. Raman,Vijayalakshmi.Kondaveeti International Journal of Life Sciences Research ISSN 2348-3148 (online) Research Publish Journals

Vol. 4, Issue 2, April 2016 – June 2016

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Extraction and Detection of Antimicrobial Compound from Streptomyces Filamentosus by Sujatha.Taidala, P.Sivaraagini, M.V. Raman,Vijayalakshmi.Kondaveeti