Minimum Exposure Time of Light-Emitting Diode (Led) Light Curing Machine in Reducing Class I Cavity Preparation Microbial Contaminants

Mark Cameron J. Acub, Tracy-Anne C. Azarcon, Charmanne Love Joy D. Go, Ramon Lorenzo G. Guerrero, Ransley Marie J. Reformado, Chryslynne L. Suganob

Abstract: Cavity medication or disinfection is a common procedure after cavity preparation in order to remove debris and reduce microbial contaminants in the preparation before placement of any restorative material. However, the oral antiseptic solutions commonly used for this procedure potentially cause either irreparable pulpal damage or staining. Light Emitting Diode (LED) light at 405 to 525 mm wavelengths has been shown to exert antimicrobial effect. This study determined the minimum exposure time for Light Emitting Diode (LED) light curing machine to reduce microbial contaminants on Class I cavity preparation. It aimed to compare the number of microbial contaminants before and after twenty, thirty, forty- second exposure to 480 nm wavelength LED light, using plate count; and to compare the relative decrease of microbial counts in the different exposure periods. Thirty teeth with Class I cavity preparations were included in the study, each exposure time having 10 samples. Right after a Class I cavity was prepared, pre-treatment microbial samples were obtained from the disto-pulpal angle after LED light exposure for 20, 30 and 40 seconds. All swabs were inoculated onto agar, incubated for 24 hours and plate counted. Results showed that microbial counts from the cavity preparations were generally lower after LED light treatment in all exposure times. Using one-tailed Student’s t-test, the lowest exposure time that showed a significantly lower number of microbial count was at 30 seconds (mean = 51.29, SD = 67.98, p = 0.046), with an average percent reduction equal to 53.5 %. Results suggest that the minimum time required for the LED light curing machine to reduce microbial contaminants in Class I cavity preparation contaminants was at 30-second exposure. The researchers recommend that the LED light curing machine be used as an alternative method of cavity disinfection to using chemical substances.

Keywords: led light, cavity debridement.

Title: Minimum Exposure Time of Light-Emitting Diode (Led) Light Curing Machine in Reducing Class I Cavity Preparation Microbial Contaminants

Author: Mark Cameron J. Acub, Tracy-Anne C. Azarcon, Charmanne Love Joy D. Go, Ramon Lorenzo G. Guerrero, Ransley Marie J. Reformado, Chryslynne L. Suganob

International Journal of Thesis Projects and Dissertations (IJTPD)

Vol. 12, Issue 1, January 2024 - March 2024

Page No: 66-71

Research Publish Journals

Website: www.researchpublish.com

Published date: 26-February-2024

DOI: https://doi.org/10.5281/zenodo.10705507

Vol. 12, Issue 1, January 2024 - March 2024

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Minimum Exposure Time of Light-Emitting Diode (Led) Light Curing Machine in Reducing Class I Cavity Preparation Microbial Contaminants by Mark Cameron J. Acub, Tracy-Anne C. Azarcon, Charmanne Love Joy D. Go, Ramon Lorenzo G. Guerrero, Ransley Marie J. Reformado, Chryslynne L. Suganob