Optimization of Concentration of Two Different Nitrogen Sources to Enhance Biofuel Potential of Chlorella Pyrenoidosa

Ramandeep Kaur, Anupama Mahajan, Anjana Bhatia

Abstract: In current scenario ‘Oilgae’ have been used as a promising source for production of biofuel. Chlorella is one of widely used microalgae for energy production and human consumption. The present study investigated the effect on biomass and lipid accumulation in Chlorella pyrenoidosa by cultivating in Fogg’s media using ammonium chloride and urea as nitrogen source. The result revealed that as concentration of N-source in media has been increased the biomass content increased but lipid content decreased and vice versa. Furthermore, at same concentration of ammonium chloride and urea, the lipid and biomass content was recorded higher in urea in comparison to ammonium chloride. The optimum concentration of urea which gave maximum biomass of 5.467 ±0.034mg/ml was 0.5g/L and maximum lipid content of 30.2 ±0.20% dry weight was recorded at 0.1g/L. At high concentration of ammonium chloride growth rate decreased due to ammonium toxicity in algal culture. The optimization of urea concentration could be proved a new cultivation strategy to enhance biofuel potential of Chlorella pyrenoidosa.

Keywords: Biofuel, Chlorella pyrenoidosa, Microalgae, Urea, Biomass, Lipids.

Title: Optimization of Concentration of Two Different Nitrogen Sources to Enhance Biofuel Potential of Chlorella Pyrenoidosa

Author: Ramandeep Kaur, Anupama Mahajan, Anjana Bhatia

International Journal of Life Sciences Research

ISSN 2348-313X (Print), ISSN 2348-3148 (online)

Research Publish Journals

Vol. 6, Issue 3, July 2018 - September 2018

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Optimization of Concentration of Two Different Nitrogen Sources to Enhance Biofuel Potential of Chlorella Pyrenoidosa by Ramandeep Kaur, Anupama Mahajan, Anjana Bhatia