Abstract: The study was aimed at optimizing the production of lipase using media and environmental parameters. Serratia GMS16 was isolated from a domestic waste dump soil sample, and evaluated for lipase producing capability. Media (carbon, nitrogen) and environmental (pH, temperature, and fermentation time) parameters were investigated for maximum lipase production. Results obtained revealed that olive oil was the best carbon source for the elaboration of lipase by the selected bacterium, however spent frying oil was adopted for production from the viewpoint of production economics and raw material availability. The inclusion of proteose peptone in the medium resulted in high lipase production. Enzyme activity was observed to increase with increasing fermentation time, at least within the ambience of the total fermentation time of 72h and at a pH of 7.0. Analysis of variance (ANOVA) of data and regression analysis showed a significant (P = 0.000; R2 = 0.958) 95.8% of final lipase concentration of 90.74 µg/mL was obtained from the interactive effects of the input variables used in the experiment.
Title: Optimization of Media and Environmental Parameters for Lipase Production by Serratia sp. GMS16
Author: Uduak Camilus Ben, Dominic Reuben Tiku, Godwin Egbe John
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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