Optimization of the Extraction of Polyphenols from Green Tea (Camellia sinensis)

Raksha.K, Roshini.TJ, Chandra Prabha.D, Dhinek.A

Abstract: Tea, the most popular beverage consumed is widely cultivated in India and China. Of the different kinds of Tea about 20% of Green Tea is being harvested annually. Green tea has gained popularity due to its remarkable health benefits. People who have consumed Green Tea were less likely to develop type-II diabetes. About 200 bioactive compounds are present in Green Tea of which,the most active are the polyphenols. Green Tea has 8000 polyphenolic compounds of which EGCG(Epigallocatechingallate) is more important (8-12% of total polyphenols). The present study focus to extract maximum amount of polyphenol by Folins Ciocalteau method for optimizing the extraction time and temperature and further utilize it to prevent diabetes. Aqueous extraction of Green Tea polyphenols was performed at three different temperatures 40°C, 50°C and 60°C and at three corresponding time 15 minutes, 30 minutes and 60 minutes. From the present study, maximum polyphenols content was obtained at 40°C for 30 minutes. Keywords: Polyphenol, EGCG, Extraction, Bioparameters, Green Tea. Title: Optimization of the Extraction of Polyphenols from Green Tea (Camellia sinensis) Author: Raksha.K, Roshini.TJ, Chandra Prabha.D, Dhinek.A International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Research Publish Journals

Vol. 5, Issue 1, January 2017 – March 2017

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Optimization of the Extraction of Polyphenols from Green Tea (Camellia sinensis) by Raksha.K, Roshini.TJ, Chandra Prabha.D, Dhinek.A