Abstract: Prevalence of laccase (LAC) producing soil fungi and bioremediation potentials of LAC crude extracts on phenol-polluted soil were assessed. LAC producing isolates screened with mineral salt agar (supplemented with 0.02% guaiacol) at 30 0C for 7 days and propagated in potato dextrose broth for 14days prior soil treatment. Twelve opaque plastic buckets were loaded with 1 kg of farm soil each and grouped into 4 sets (triplicate per set). Two sets designated treated soils were TS1 and TS2, and two sets as control soils (CS1 & CS2). TS1 and TS2 polluted with 100 mL and 150 mL of phenol respectively, were treated with 100 mL of LAC crude extracts and compared with untreated soils CS1 (100 mL phenol) and CS2 (0 mL phenol) within 5 weeks. Among the isolated fungi, only 62% were LAC producers (Fusarium sp, Absidia sp and Rhizopus sp). LAC treatments (TS1 &TS2) significantly reversed the adverse effect of phenol on soil pH and bioactivities (BOD and TOC) by 60-100% but insignificant to soil temperature. Phenol removal were optimized by TS2 (46%) and TS1 (21%), while natural degradation by CS2 was only 6% in 5 weeks. Thus, these findings are recommendable as cheap and ecofriendly alternative for managing industrial waste (phenol) pollutions.
Keywords: Laccase, Bioremediation, Soil fungi, optimize, Phenol.
Title: Optimizing Bioremediation of Phenol Polluted Soil Using Laccase from Soil Fungi
Author: Balogu, V. Tochukwu, Mohammed, G. Ramatu, Mohammed, S. D Sani, Yewodo, J. Clement
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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