Abstract: Calophyllum inophyllum is a large evergreen plant, low-branching and slow-growing tree with a broad and irregular crown and it is native to Africa, southern coastal India. It is best known as an ornamental plant, trees often grow in coastal regions, as well as nearby lowland forests. The oil was extracted from dried seeds of Calophyllum inophyllum using Soxhlet extraction column and used as carbon source for the growth of Cupriavidus necator. This organism was fed in batch mode and the culture was utilized for the production of Polyhydroxybutarate (PHB). The microorganism was procured from MTCC Chandigarh [MTCC No-1472]. The fermentation time for the production of PHB was observed to be 72hr. The intracellular PHB from the cells was extracted using various organic solvents and analyzed using Fourier Transform Infra-red spectroscopy [FTIR] and the peaks are compared with the standard peaks.
Title: Production Of PHB From Cupriavidus Necator Using Calophyllum inophyllum seed oil as Carbon Source
Author: Likitha reddy.V, Namratha.V, Dr.Sarada.B
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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