Abstract: In this study, a section of the human CDKN2A gene was obtained from unknown individuals and analyzed for the purpose of detecting any probable single nucleotide polymorphisms (SNP). The exon-2 of this gene was chosen because its mutation was previously reported to be associated with malignant melanoma. At first, suitable primers were designed for a specific region of the CDKN2A gene including exon-2 and later the area was amplified several times at different temperatures through polymerase chain reactions (PCR). The PCR products of 60°C with 250ng human placental DNA was extracted for quantification and sequencing. After the sequencing, the three CDKN2A gene transcripts were found to be a 100% match with the database in NCBI and. no SNP was detected. However, in the genomic sequences, an insertion of nucleotide was detected in the intron of this gene. Furthermore, protein translation in 5’-3’ open reading frame was significant.
Keyword: CDKN2A, SNP, malignant melanoma, PCR.
Title: The Human CDKN2A Gene: Analyzing How Mutations of a Section of the Gene Can Be Associated with Malignant Melanoma
Author: Md. Anisur Rahman, Abdullah Al Maruf, Dr. Repon Kumer Saha, Ivor H. Evans
International Journal of Life Sciences Research
ISSN 2348-3148 (online), ISSN 2348-313X (Print)
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