Abstract: PRC is a part of the male reproductive system that helps make and store seminal fluid. In adult men, a typical prostate is about 3 centimetres long and weighs about 20 grams. It is located in the pelvis, below the urinary bladder and in front of the rectum. Most of the nRTKs are localised in the cytoplasm. TNK1/Kos1 is a 72-KDa NRPTK located on the human chromosome 17p13.1. TNK1/Kos1 is a member of the Ack family. The main function of non-receptor protein-tyrosine kinase (nRTKs) is to participate signal transduction in activated T-cells and B-cells in the immune system. To investigate the role of TNK1 in PC-3 cells, TNK1 deficient cells were used in the study. The results showed that TNK1 deficiency, migration and invasion were enhanced in the absence of functional TNK1. Immunoblotting results revealed decreased Akt phosphorylation level and increased Erk phosphorylation level. These results suggest that TNK1 might contribute to the maligancy of tumor cells, therefore, TNK1 might be a potential prognosis factor for metastatic prostate cancer.
Keywords: PRC / TNK1 / Migration / Invasion / PC-3 cells.
Title: TNK1 Deficiency Promoted Cell Migration, Invasion Assay and Effects on MAPK and PI3K/Akt Signalling Pathways
Author: ALAULDDIN HAZIM MOHAMMED
International Journal of Life Sciences Research
ISSN 2348-313X (Print), ISSN 2348-3148 (online)
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