Vol 2 Issue 2 October 2014-March 2015

Abstract: Point of care testing (POCT) refers to testing that is performed near or at the site of a patient with the result leading to a possible change in the care of the patient. Although there are many benefits of using POCT devices in terms of their convenience, establishing a POCT indeed is a challenging job. Some of the biggest challenges for the person holding this post are gaining physician and nurse allies and turning non-laboratorians into testing personnel, all while ensuring adherence to best laboratory practices and regulatory agency standards. POCT implementation requires a systematic approach which involves all stakeholders. Most of the healthcare organizations are unfamiliar with the POCT due to which very less efforts are made to establish a complete POCT set up.

Keywords: Location, Devices, Quality Control, Data Management, Training, Proficiency.

Title: CHALLENGES IN POCT

Author: Dr. Jayesh P. Warade

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: Sample of ten patients defected with medulloblastoma and rhabdomyomas are selected to treat by heavily armed vector (Epstein Barr Virus with catalase enzyme supported with long chain of phosphate group synthesized in self funded laboratory with esterase enzyme).

Ten samples of patients were invited from Jordan University of science and technology university hospital and king Hussein cancer center of these ten samples five defected with primitive neuroectodermal tumors (medulloblastoma) and the other five defected with primary tumors in infants and children of heart (rhabdomyomas). nine of ten were selected to expose to catalase enzyme of one to six genes of the vector capsid envelop protein and it takes seven days; the last is exposed to insertion of phosphate group chain inside tumor cells by stereotactic microinjection armed (EBV) by this group inside brain tumors (medulloblastoma) and primary heart tumors (rhabdomyomas),other ten exposed directly forward to acetyl esterase gene by viral vector instead of seven and eight genes which are defected genes for human as considered other expose to three stages with effectively potential Armed vector of medulloblastoma and rhabdomyomas.

This study showed significantly prolonged median survival time of 10 days for the first exposing of armed catalase enzyme in order to get catalysis procedure inside tumors itself when it reacts with c-AMP then to destroy both tumors (case of control inside tumor cells and the reagent is PCR) which can indicate the tumor controlling when the bands get the same size on gel electrophoresis 95 Kbp; the second case is using long armed chain p-group which allow to help catalase enzyme to get catalysis procedure inside brain and heart then it will react with c-AMP to destroy SHH and NOTCH genes in medulloblastoma in the brain and rhabdomyomas in the heart and it takes 25 days. the final result takes 27 days and it started from the beginning of consisting 3 factors which are 1-catalaseenzyme located from gene 1 to 6 of the vector 2-phosphate group inside tumor cells via vectors 3-estrase gene. all of these genes collected together in a single chain with 5˞-p-group to construct a strong Armed vector to do as a surgical neuroonclogist in the operating room of primitive neuroectodermal tumor and primary heart tumor in infants and children to remove fully seized of tumors in both cases which has 10 patients of them by acting as precursor of c-AMP to fully destroyed SHH and NOTCH genes without altering the expression of 5ʹ-p-group; catalase enzyme; esterase enzyme in the tumor cells.

We conclude that Epstein Barr Virus which Armed by catalase gene; 5prime-p-group; esterase gene solved the biggest problem in the world medulloblastoma and rhabdomyomas in tumors in cerebellum and heart of infants and children without any toxic side effect of both diseases treatment. The prolonged survival time for 10 samples receiving this dose by Armed vector appeared that no neurological symptoms in both tumors brain and heart.

Abbreviations: ATA – replication incompetent adeno associated vector carrying the EBV EST gene – CAT gene and Armed 5 prime p group; BSA – bovine serum albumin; CSF – cerebrospinal fluid; EGF – epidermal growth factor; EGFR – epidermal growth factor receptor; ASR – arc single strand; EBV – Epstein Barr Virus; PBS-phosphate buffer saline; RT – room temperature; EST – esterase gene; CAT – catalase gene; 5 PRIME – P – GROUP – long chain of synthesized phosphate group; HALS – helicase gene; TNF α – tumor necrosis factor alpha; VP – vector particles; *EBV-FUNJAN1* - Epstein Barr Virus 7 and 8 genes.

Keywords:  Epstein Barr Virus, medulloblastoma, long chain of phosphate group, esterase gene, rhabdomyomas, *EBV-FUNJAN1* genes.

Title: Armed Epstein Barr Virus Gene Therapy of Human Medulloblastoma Clinical Trial Phase-II (100% Powerful & Fully Treated Vaccine)

Author: Leen Funjan, Iman Funjan, Yara Funjan, Areen Funjan, Ahmad Funjan

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: Primary hydatid disease in the muscle is very rare. Hydatid cysts develop most frequently in the liver and lungs, but they are occasionally found in other organs. Hydatid cysts in the neck are an extremely rare event in areas where the disease is endemic. Here, we report our experienceto conclude the fact that hydatid cyst should be included in the differential diagnosis of cystic mass to avoid   investigations like FNAC because the rupture of cyst may not only result in its complete surgical resection is difficult but also there is risk of anaphylaxis, recurrence and dissemination to other sites.

Keywords: Echinococcosis, Intramuscular, Hydatid cyst.

Title: Primary Soft Tissue Hydatid Cyst in Neck: A Case Report and Review of Literature

Author: Dr. Rajesh Lonare, Dr. Pradeep Balmiki, Dr. Sanjay Parashar, Dr. Pradyumn Gupta, Dr. Shantanu Talla

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: A 45 year old male patient diagnosed case of chronic myeloid leukemia (CML) in 2003 being treated with dasatinib since 2010 came to the emergency department with complaints of fever, cough and breathlessness. On examination bilateral pedal edema was present and chest X-ray showed right sided pleural effusion. Pleural tap has been done with which his breathlessness was improved. Patient was initially started on antibiotics but not responded to the therapy. Now pleural effusion was considered as dasatinib induced and started on a trial of low dose oral prednisolone. Then patient shown improvement dramatically and successfully treated with short course of low dose oral prednisolone.

Keywords: Imatinib resistant, chronic myeloid leukemia, tyrosine kinase inhibitors, pulmonary arterial hypertension.

Title: DASATINIB INDUCED PLEURAL EFFUSION TREATED WITH LOW DOSE PREDNISOLONE

Author: Dr. Kavitha Saravu, Dr. Pradeep Kumar Reddy Sura, Dr. Barkur A. Shastry, Dr. Ajit Singh

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: High resolution cross sectional imaging dramatically improves the diagnosis and therapy of oncological patients primarily concerning the exact initial staging, differential diagnosis of recurrent tumours and therapy management. Positron emission tomography (PET) is a quantitative, functional diagnostic imaging modality using compounds labelled with positron-emitting radioisotopes to measure cell metabolism for both initial tumor staging and detection of tumour recurrence with a high diagnostic accuracy. Combined PET/CT is a unique imaging modality that permits anatomic and functional imaging on a single scan in comparison with PET alone. Nearly a decade after introduction of PET/CT, clinical combined PET/magnetic resonance imaging (PET/MRI)  systems have become available commercially. PET/MRI system combines the unique features of MRI including excellent soft tissue contrast. Thus this paper portrays the usefulness of PET along with its recent advances in head and neck cancers.

Keywords:  PET scan, FDG, Metastasis.

Title: Application of Positron Emission Tomography in Head and Neck Cancer - An Overview

Author: N.Vijayalakshmi, Dr.Indira.A.P, Dr.Maria Prisicilla David

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: The present investigation was carried out on 40 college going unmarried girls between the age group of 18-21 years having menstrual disorders in Pilani town of District Jhunjhunu to study the effect of dietary habits on premenstrual syndromes, menstrual disorders and practices related to menstruation among college going girls. Results indicate that majority of respondents reported restriction and taboos associated with menstrual period and dietary habits. Common disorders of menstruation among respondents were dysmenorrhoea, menorrhagia, irregular menstruation, hypomenorrhoea, lecomenorrhoea. Menstrual syndromes experienced were headache, abdominal pain, acne, loss of appetite, cramps, body ache and fever by the respondents. High fat and low fibre diet was reported which resulted in menstrual problems among them. There were differences in the appetite during premenstrual and postmenstrual phases. Increased appetite was seen at the premenstrual phase and low intake of fibre, vitamin A, vitamin C, calcium and iron showed adverse effects on health of the respondents during premenstrual phase.

Keywords: Adolescents, menstrual disorders, practices, dietary habits, nutrition.

Title: To Study the Effect of Dietary Habits on Premenstrual Syndromes, Menstrual Disorders and Practices Related To Menstruation among College Going Girls.

Author: Shaifali Pathak

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals

Abstract: Flabby ridges commonly occur in edentulous patients were inadequate retention and stability of a complete denture are the often encountered. A liquid supported denture due to its flexible tissue surface allows better distribution of stresses and hence provides an alternate treatment modality in such cases. A liquid-supported denture was planned for maxillary arch with neutral zone concept for fabrication of contour of the polished surfaces of mandibular denture.  This case report presents the use of a liquid supported denture in a patient with completely edentulous maxillary arch with flabby tissue in anterior region opposing neutral zone concept for atrophied edentulous mandibular ridge.

Keywords: Flabby ridges, edentulous patients, Denture & Neutral Zone technique.

Title: Management of flabby and atrophied ridges using Liquid-Supported Denture & Neutral Zone technique: A Case Report

Author: Dr. Gunjan Gupta, Dr. Varun Goyal, Dr. Anshul Mel, Dr. Alpa Gupta, Dr. Anil Kadian

International Journal of Healthcare Sciences

ISSN 2348-5728 (Online)

Research Publish Journals